Commercial enzyme-linked immunosorbent assay for neopterin detection in blood donations compared with RIA and HPLC.

Sensklvity Fig. 1. ROC curves constructed from initial results by RIA (-) and EUSA (-) for 109 blood donors who were classified into two groups according to HPLCneoptenn cutoff of less than orequal to,ormore than, 10 nmol/L Sensitivity, fraction of subjects with HPLC >10 nmol/L whose initial AlAor EUSA result was above a chosen cutoff value. Specificity, fraction of subjects with HPLC values 1O nmol/L whose initial AlA or EUSA result was below or equal to a chosen cutoff value. The cutoff values were varied systematically over the whole range of values observed by RIA or ELISA.The greaterthe area under the ROC curve, the better the diagnostic power of the test under consideration.